|
KMID : 1134820000290040719
|
|
Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 4 p.719 ~ p.725
|
|
|
Purification and Characterization of Angiotensin I - Converting Enzyme Inhibitor from Porphyra yezoensis
|
|
Choi Soo-Jin
Jun Woo-Jin
Yoo Kwang-Won
Shin Dong-hoon
Hong Bum-Shik
Cho Hong-Yon
Yang Han-Chul
|
|
|
Abstract
|
|
|
|
This study focused on the purification and characterization of ACE inhibitor from Porphyra yezoensis. The dried Porphyra yezoensis was ground and hydrolyzed with 2.5 N HCl, followed by neutralization and centrifugation. Then, the subsequential purification of ACE inhibitor was carried out by Amberlite XAD 8, DEAE-Toyopearl 650C, Sephadex LH-20 column chromatography and reverse phase HPLC with C_(18) column. The purified ACE inhibitor was peptide which consisted of glycine (24.5%), arginine (56.8%) and proline (18.8%). Also, it showed the competitive inhibition pattern to ACE. The apparent molecular mass of purified peptide was 580 dalton, and an IC50 value of ACE inhibitor was 10.6 μg.
|
|
|
KEYWORD
|
|
|
angiotensin I-converting enzyme (ACE), peptide, Porphyra yezonensis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|